Journal: International Journal of Molecular Sciences

Article Title: How Is Arachidonic Acid Metabolism in the Uterus Connected with the Immune Status of Red Deer Females ( Cervus elaphus L.) in Different Reproductive Stages?

doi: 10.3390/ijms24054771

Figure Lengend Snippet: mRNA expression of 5-LO ( A ), LTA4H ( B ), LTC4S ( C ), PTGS2 ( D ), PGES ( E ), PGFS ( F ), and PGIS ( G ) in uterine tissues (endometrium and myometrium) on 4th and 13th day of the estrous cycle, and in pregnancy and anestrus phase. Data were normalized against GAPDH for mRNA expression using GraphPad PRISM (Version 8.3.0). Each bar represents one experimental group with SEM. Statistical differences were analyzed withy two-way analysis (ANOVA) of variance followed by Tukey’s post hoc test. The lowest statistical significance was p < 0.05. Asterisks indicate statistical differences between endometrium and myometrium (* p < 0.05; ** p < 0.01; *** p < 0.001). Different letters indicate statistical differences ( p < 0.05) between the experimental reproductive stages throughout endometrium (a, b) and myometrium (A, B), respectively.

Article Snippet: Afterward, the proteins were transferred to 0.2 μm nitrocellulose membranes in transfer buffer by electroblotting for 1.5 h. The membranes were blocked in a 5% solution of skimmed milk with 1x TBS-T for 1.5 h at room temperature (RT), and then incubated overnight at 4 °C with specific primary antibodies for 5-LO (Cayman, 160402, 1:1000), LTA4H (Cayman, 160250, 1:1000), LTC4S (Biorbyt, orb580627, 1:1000), PTGS2 (Cayman, 160107, 1:1000), PGES (Cayman, 160140, 1:1000), PGFS (Cayman, 10007940, 1:1000), and PGIS (Cayman, 160640, 1:1000).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: How Is Arachidonic Acid Metabolism in the Uterus Connected with the Immune Status of Red Deer Females ( Cervus elaphus L.) in Different Reproductive Stages?

doi: 10.3390/ijms24054771

Figure Lengend Snippet: Protein expression of 5-LO ( A ), LTA4H ( B ), LTC4S ( C ), PTGS2 ( D ), PGES ( E ), PGFS ( F ), and PGIS ( G ) in uterine tissues (endometrium and myometrium) on 4th and 13th day of the estrous cycle, and in pregnancy and anestrus phase. Data were normalized against ACTB for protein expression using GraphPad PRISM (Version 8.3.0). Each bar represents one experimental group with SEM. Statistical differences were analyzed with two-way analysis (ANOVA) of variance followed by Tukey’s post hoc test. The lowest statistical significance was p < 0.05. Asterisks indicate statistical differences between endometrium and myometrium (* p < 0.1; *** p < 0.001). Different letters indicate statistical differences ( p < 0.05) between the experimental reproductive stages throughout endometrium (a, b) and myometrium (A, B), respectively.

Article Snippet: Afterward, the proteins were transferred to 0.2 μm nitrocellulose membranes in transfer buffer by electroblotting for 1.5 h. The membranes were blocked in a 5% solution of skimmed milk with 1x TBS-T for 1.5 h at room temperature (RT), and then incubated overnight at 4 °C with specific primary antibodies for 5-LO (Cayman, 160402, 1:1000), LTA4H (Cayman, 160250, 1:1000), LTC4S (Biorbyt, orb580627, 1:1000), PTGS2 (Cayman, 160107, 1:1000), PGES (Cayman, 160140, 1:1000), PGFS (Cayman, 10007940, 1:1000), and PGIS (Cayman, 160640, 1:1000).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: How Is Arachidonic Acid Metabolism in the Uterus Connected with the Immune Status of Red Deer Females ( Cervus elaphus L.) in Different Reproductive Stages?

doi: 10.3390/ijms24054771

Figure Lengend Snippet: List of monoclonal antibodies used for labeling lymphocytes for flow cytometry.

Article Snippet: Afterward, the proteins were transferred to 0.2 μm nitrocellulose membranes in transfer buffer by electroblotting for 1.5 h. The membranes were blocked in a 5% solution of skimmed milk with 1x TBS-T for 1.5 h at room temperature (RT), and then incubated overnight at 4 °C with specific primary antibodies for 5-LO (Cayman, 160402, 1:1000), LTA4H (Cayman, 160250, 1:1000), LTC4S (Biorbyt, orb580627, 1:1000), PTGS2 (Cayman, 160107, 1:1000), PGES (Cayman, 160140, 1:1000), PGFS (Cayman, 10007940, 1:1000), and PGIS (Cayman, 160640, 1:1000).

Techniques: Labeling, Cytometry, Sequencing, Amplification

Journal: International Journal of Molecular Sciences

Article Title: How Is Arachidonic Acid Metabolism in the Uterus Connected with the Immune Status of Red Deer Females ( Cervus elaphus L.) in Different Reproductive Stages?

doi: 10.3390/ijms24054771

Figure Lengend Snippet: Oligonucleotide sequences used for real-time PCR. GAPDH—Glyceraldehyde 3-phosphate dehydrogenase, RN18S1—18S ribosomal RNA, ACTB—β-actin, 5-LO—5-Lipoxygenase, LTA4H—Leukotriene A4 hydrolase, LTC4S—Leukotriene C4 Synthase, PTGS2—prostaglandin endoperoxide synthase 2, PGES—Prostaglandin E2 synthase, PGFS—Prostaglandin F2alpha synthase, PGIS—Prostaglandin I2 synthase.

Article Snippet: Afterward, the proteins were transferred to 0.2 μm nitrocellulose membranes in transfer buffer by electroblotting for 1.5 h. The membranes were blocked in a 5% solution of skimmed milk with 1x TBS-T for 1.5 h at room temperature (RT), and then incubated overnight at 4 °C with specific primary antibodies for 5-LO (Cayman, 160402, 1:1000), LTA4H (Cayman, 160250, 1:1000), LTC4S (Biorbyt, orb580627, 1:1000), PTGS2 (Cayman, 160107, 1:1000), PGES (Cayman, 160140, 1:1000), PGFS (Cayman, 10007940, 1:1000), and PGIS (Cayman, 160640, 1:1000).

Techniques: Real-time Polymerase Chain Reaction